FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41705513
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35300.013.44808126
ATATAGC42450.013.3033536
GTCGTTC41150.011.97355729
GGAGGTC233600.011.6575384
GGGAGGT244400.011.4462773
TGTCGTT45100.010.97478728
ATAGGGG197550.010.82046738
GGTCACT241800.010.7271517
GTCACTC243950.010.7242868
GCTAAGT48450.010.6812514
GGCATAG192550.010.64535735
AGGTCAC263300.010.236966
CGGCTTA65050.010.02993330
AGCTTAG207250.09.99849945
ATATCGT47300.09.60872533
GTTTCGT58650.09.5929741
TGGCATA219750.09.47093934
TTCGTCG54100.09.4432813
AATATAG72850.09.2988935
TCGTTCC51100.09.24582630
Produced by FastQC (version 0.11.5)