FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48178761
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA94900.021.71717323
ACGCTAC96100.021.30551524
CGCTACC100700.020.22055825
GTCCGTT32850.019.188621
TCACGCT116100.018.914328
AAAACGC113550.018.2889821
GAAAACG114650.018.19200520
CGCAATC53450.017.7229272
TACGGTA105350.017.53402944
CACGCTC125750.017.4986279
TTAACGC28750.017.3735377
ATTAACG29500.017.2379516
ATGCGCG41450.017.15289745
ACGTTCG22950.016.9614376
GCCGTAT43500.016.8713341
ACCGCGT59250.016.7854586
ACGCTCA131650.016.74859210
CGATTAG48650.016.4189264
AGACCGC61600.016.2546844
CGTATCG40400.015.9845453
Produced by FastQC (version 0.11.5)