FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52326926
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG631870.1207542747685962No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT549420.10499756855581388No Hit
CCCTCGTCCAACCAGCTGCCACGTCCTGTAACCAAAAGTGACCGGGATGAA537680.10275398176456993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG131100.020.9986465
ACCGCGT133200.020.7013366
AGACCGC139500.019.9116944
CCGCGTT140200.019.7158727
CGCGTTC141050.019.6608418
TCACGCT196850.019.3662918
GCCGGCT176000.019.3387891
CCGGCTT208400.019.016562
TTCACGC200650.018.9322667
AACGCTA196400.018.67846523
ACGCTAC198300.018.52232624
CAGACCG152400.018.4330983
CGCTACC204150.017.92547425
ACGCTCA219250.017.8699110
AAAACGC213450.017.36533421
GAAAACG221800.016.7621820
CACGCTC229500.016.7189949
CGGCTTC258050.016.063583
GAACGAT174900.015.93037545
CTACACG87700.015.88496245
Produced by FastQC (version 0.11.5)