FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47778038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT754190.15785286118278863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC179800.019.8723474
ACCGCGT181550.019.7423236
GACCGCG183050.019.5436735
CAGACCG188150.019.074773
CCGCGTT199500.017.9433787
CGCGTTC204400.017.5022228
TTCGACG18200.016.9366666
TAAACCG74150.016.3551511
TCAGACC226900.016.0467592
GTCCGTT34050.015.9957391
GAACGAT230100.015.43004945
GCGTTCT236450.015.272599
CTCGCGT7850.015.1954041
CTCCGGT60850.015.0165961
AACCGAT81900.014.94486313
GCACTAC78700.014.66631325
CCGGCTT179200.014.6044432
TTCACGC160250.014.2509877
CGTTACG18950.014.24786821
CCCTAAC28350.014.2103911
Produced by FastQC (version 0.11.5)