FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47778038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG655600.1372178572925075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC331150.013.3876564
GGGAGGT341500.013.1400643
GTTGTCG27600.012.71631326
ATAGGGG246100.012.415750538
AGCTTAG251950.012.26120845
GGCATAG251100.012.08733335
GTCACTC364000.012.0657078
GGTCACT365400.011.9034087
GAGGTCA390900.011.7033265
TAGCACG20150.011.27752629
ACTCTCC393900.011.22963111
AGGTCAC409150.011.1315246
TGGCATA275500.011.07406234
GCGTCGT19750.011.05026311
TCACTCT404450.010.9642839
GAGCTTA289250.010.71940544
CACTCTC421650.010.47423910
CCGCGTT28050.010.3474113
CGCGTTC27200.010.33994414
ACCCGGG90550.010.1398192
Produced by FastQC (version 0.11.5)