FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50900542
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA120000.019.16223345
ATCGCGT17000.018.3968936
CTCGACG133050.017.29964644
TAAACCG135500.017.16950211
ATCGAGC89250.016.15943742
GATCGCG19650.016.1449415
CTCCGGT74800.016.0673921
GACCGCG66800.015.7296955
AACCGAT149050.015.71430813
AGACCGC67600.015.7104734
CTCCGAT60100.015.6908071
CGTTACG16500.015.54528321
AAACCGA154200.015.23325212
CCCAAAT229200.015.1908291
ACCGCGT69800.015.1180596
TCTCGAC155850.014.85542443
GTCCGTT40300.014.855331
CGAGCTT101250.014.71090744
CGCGTTC93300.014.6139458
CTCGTAT81900.014.537091
Produced by FastQC (version 0.11.5)