FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50796211
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT85500.020.22551345
ACGTTCG17050.019.9239526
AGACCGC88900.019.359124
TCGACGA126250.019.26179545
GACCGCG89350.019.1104745
ACCGCGT89650.019.0213726
GTCCGTT45400.018.8817041
CTCGACG132650.018.468944
CAGACCG96500.018.137553
CCGCGTT95450.017.8184017
CGCGTTC96300.017.6611988
ATCGAGC83350.016.93242542
TAAACCG145900.016.89978411
TATTCGC83800.016.59091817
TTATTCG86150.016.1383516
TCGGCGC54850.016.01003845
AACCGAT159850.015.45308513
GACGAAA53300.015.25051145
AATACGG114550.015.12409732
CCCAAAT217950.015.1131941
Produced by FastQC (version 0.11.5)