FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48434102
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA701390.14481325575108214No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACTTCTCTTCCTCGTGGTTTTCG531920.10982344629823011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34300.023.81186
GACCGCG50100.020.6586555
GTTCGAC40700.020.3439545
ACCGCGT50850.020.3096056
CGACGAG39400.020.2726928
AGACCGC51450.019.9419124
CGCAATC39450.019.2788942
CTCCGGT63700.018.583591
CCCGTAT31200.018.2494261
CCGGCTT322800.017.9426862
CAGACCG59350.017.4395833
CCCCCCT38150.016.9895251
TTCACGC321800.016.8364187
GTCCGTT45300.016.7422941
GCCGGCT289250.016.7126061
CGCCTGT44200.016.7007061
TCACGCT324800.016.6047088
CAGTTCG54000.016.4589583
CCCGGCT85050.016.4323831
TACGCGA1703.4156983E-815.8822348
Produced by FastQC (version 0.11.5)