FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48434102
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG901760.18618286760018798No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA672090.13876379910997422No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG518250.10700105475270297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC352500.016.2113864
GTTGTCG48700.016.1693926
ATATAGC60250.015.6090656
GGGAGGT375500.015.3442823
AGCTTAG313550.014.44440345
GGCATAG318950.014.08697235
ATAGGGG321700.014.03104638
GTCACTC403150.014.0174778
GGTCACT409400.013.8045517
TGTCGTT58650.013.50339928
GCTAAGT60150.013.46574514
AGGTCAC433650.013.3959836
GTCGTTC59450.013.32164729
GAGGTCA447100.013.229125
ACCCGGG72600.013.0515482
TGGCATA350700.012.94673234
GAGCTTA362200.012.59175744
ACTCTCC453000.012.50006311
CACCCGG79250.012.4959311
TCACTCT456050.012.4555289
Produced by FastQC (version 0.11.5)