FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46942242
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA616550.13134225672476402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG121550.012.45201738
GTTCTAT264900.012.4129551
GGCATAG126850.011.890326535
CACCGAA300000.011.5226445
GCACCGA298800.011.2824944
TTCTATA317500.011.141032
AGCTTAG141500.011.00605645
ATAGTGT319200.010.8596446
TAGTGTG318100.010.7911477
CTATAGT316400.010.7803994
GTCACTC199700.010.7277128
CATAGGG143000.010.72512137
TCTATAG326350.010.6315423
TATAGTG324750.010.5783465
GGAGGTC214000.010.5068364
GTTGTCG20650.010.44951726
GGTCACT204200.010.4032337
TTACTAG327250.010.2819114
TACTAGA325600.010.278789515
TAGAGCA344300.010.22645540
Produced by FastQC (version 0.11.5)