FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45706983
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGT72100.016.8582841
AGACCGC52350.015.0435184
GAACGAT52900.014.84545245
TCGGCGC40850.014.70763645
ACCGCGT55450.014.4848766
CCCGTAT32250.014.3777981
CCCCCCT56900.014.280711
GACCGCG56400.014.1627555
TCGTGCG26300.014.11784841
GTCCGTT35350.013.9447131
CAGACCG59550.013.4517593
TTCGGCG45850.013.15313144
CGTACGC46950.013.0835495
CCCCCGT52850.013.0326071
CCCCGCT116050.012.8982671
CGTTACG18650.012.78727521
CCGCGTT62650.012.71248157
GTCCGCT43300.012.6840161
TACCCCG30200.012.66450518
CCCCCTT134350.012.6324771
Produced by FastQC (version 0.11.5)