FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49583914
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG82750.011.474380538
TAGCACG23250.011.12851729
GGCATAG85500.010.84139535
CGGCTAA105000.010.71403931
CGGCTTA67250.010.53869530
CATAGGG95050.09.94171637
GTTTCGT55700.09.8179821
GTTGTCG20050.09.65001826
CGGTTTA49000.09.59698938
ACGGCTA121300.09.40406730
GCATAGG99600.09.30682736
TTACGCG8300.09.21626535
ACCCGGC41050.09.2096335
TTCGTCG52750.09.0019553
TTTCGTC60300.08.8450882
CGCTGAT146650.08.7931351
AGCTTAG110200.08.57518945
TCAGACG60500.08.5182682
GTCACTC161400.08.4338978
GGTCACT156500.08.4111567
Produced by FastQC (version 0.11.5)