FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49968105
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1034830.20709810788301858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT214800.024.65585745
GACCGCG239000.021.8659175
AGACCGC243450.021.706214
CAGACCG249850.021.2492393
ACCGCGT248400.021.0928026
CCGCGTT259800.020.2016477
CGCGTTC261850.020.0606738
TTCGACG17850.019.7869036
AGAACGA273200.019.48293344
TCAGACC288050.018.9700952
TCACGCT169850.018.873858
AACGCTA160900.018.8053123
ACGCTAC162150.018.74358224
AATACGG290850.018.1230732
ATACGGA292750.018.03618833
TTCACGC178150.017.9818927
GCGTTCT293750.017.9668459
CGCTACC169400.017.87499225
TACGGAG294200.017.8093534
AAGAACG306850.017.351243
Produced by FastQC (version 0.11.5)