FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41008148
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT522780.12748198236116393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT119000.021.42472645
AGACCGC123350.020.7412594
GACCGCG127450.020.0563665
ACCGCGT129100.019.8144156
CAGACCG131950.019.526443
ATCGAGC60850.019.41592442
TCGGCGC41050.019.18612145
CTACCGG18150.019.08895512
CCGCGTT135850.018.813477
CTCCGGT65950.018.598351
CGCGTTC140600.018.2253078
TTCGGCG45350.017.76445244
AGAACGA145650.017.48968544
TCGACGA65950.017.3674245
GTCCGTT34900.016.5084841
CTCCGTT85800.016.2628671
CGTACGC50300.016.2387435
CCGTACG51450.015.7883084
TCAGACC169450.015.4976122
TCTACCG23300.015.35250511
Produced by FastQC (version 0.11.5)