FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41008148
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG61700.011.26572738
GTTTCGT52400.011.0408761
ACCCGGC44250.010.9384025
GGCATAG65100.010.5733535
TTCGTCG50350.010.5073813
CGGCTAA53950.010.00851831
CGGCTTA58350.09.98652130
TTTCGTC56150.09.98333552
CATAGGG69350.09.79593437
CGGTTTA54700.09.4585938
ATAGCTC49150.09.2450939
TATACGC13500.09.16687413
AGCTTAG78350.09.04584445
GTCACTC117000.08.941668
GCATAGG76100.08.86789236
CGTCGAG63250.08.862735
CGGACAC54850.08.61459813
CGGCCCG61550.08.2609778
CGGACAG59200.08.24746219
CACACGC100600.08.2128554
Produced by FastQC (version 0.11.5)