FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46058911
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT751700.16320403233155034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT158850.023.45670945
AGAACGA200500.018.58435444
AGACCGC203300.018.3825594
ACCGCGT204250.018.3297866
GACCGCG204100.018.3215315
CCGCGTT213900.017.4919877
CAGACCG215400.017.4230463
CGCGTTC217700.017.207338
TCGACGA57000.016.0267445
AAGAACG238950.015.62235743
ATCGAGC55950.015.48368942
CTCCGGT52950.015.3014431
CGTTACG12800.015.292541521
CGTACGC35000.015.1711215
AATACGG249200.015.0064832
GTCCGTT34100.014.98191451
CCCGTAT30600.014.9303661
GCGTTCT253700.014.8543759
TACGGAG254700.014.68243134
ATACGGA256550.014.60286733
Produced by FastQC (version 0.11.5)