FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49802514
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG724530.1454806076657094No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT509850.10237435001775211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33050.027.6392426
CGACGAG42350.021.3564038
AGACCGC134950.020.5404224
ACCGCGT135000.020.4828176
GACCGCG144100.019.1893165
CAGACCG146500.019.0899623
CGGTATA66600.018.9515220
TCACGCT229100.018.8061188
CCGCGTT147500.018.7927287
CGCGTTC150250.018.4630748
GAACGAT154800.017.84810345
AACGCTA232000.017.35895323
ACGCTAC234000.017.22030424
CACGCTC252450.016.9062549
GCCGGCT230700.016.8980051
ACGCTCA256950.016.84658410
TAAACCG109950.016.7179211
AAAACGC246850.016.35101521
CTCGACG110400.016.34418944
TCAGACC177700.016.1940212
Produced by FastQC (version 0.11.5)