FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47855930
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2230780.466144947971965No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA648690.13555059947638673No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG557190.11643071193057997No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA550190.11496798829319585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT314650.032.73731245
AGAACGA398800.025.99890344
AGACCGC435900.023.99124
GACCGCG437900.023.912535
ACCGCGT441850.023.6681336
CAGACCG445150.023.5937143
CCGCGTT458100.022.8089167
CGCGTTC461450.022.6384538
AAGAACG464800.022.3075243
TCAGACC475400.022.2253882
ATACGGA470100.021.98712733
AATACGG471700.021.96022232
TACGGAG478650.021.57561934
GCGTTCT486800.021.4966059
CAATACG483300.021.42382831
ACGGAGA501100.020.66775935
CTCAGAC542850.020.5245321
CGTTCTC522050.020.19609810
CCAATAC529550.019.83737630
CCCCAAT539600.019.32613828
Produced by FastQC (version 0.11.5)