FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51731130
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT616400.11915455935333327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG39050.026.0454566
ACCGCGT140200.021.538686
TCACGCT217650.021.4499638
GACCGCG143350.021.081085
AGACCGC147950.020.5625154
CGCGTTC148600.020.5309018
CCGCGTT148800.020.4579457
ACGCTCA231850.020.45660610
TTCACGC234500.020.0334057
AACGCTA228500.019.91944523
CAGACCG155100.019.759683
ACGCTAC231950.019.67166524
CGGTATA59850.019.66097620
CGACGAG50600.019.6534548
CGCTACC240150.019.0093425
CACGCTC248000.018.9157529
GAACGAT168750.018.3593545
GCCGGCT164500.018.2136761
AAAACGC252950.018.14523521
GTTCGAC54450.018.1418345
Produced by FastQC (version 0.11.5)