FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46360266
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1094480.23608147546004157No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT702090.15144218542663238No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG627730.13540258806970606No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT587750.1267788239178783No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACACT496880.10717798728764844No Hit
CTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACACTTGGGGTACTT480110.10356066550610388No Hit
CCCAGAGAGAGGTAGGTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTG473440.1021219334677674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG1350.028.32510810
TACGCGC2250.028.02672644
TACGCGA1150.027.38479818
CTATGCG26300.026.8553497
GTTCGCG4450.026.7958981
ACTAGCG4000.026.4298534
CCGACTA30400.023.3814183
CGCTATA2750.022.9024642
CGCTACT8500.022.7631281
ATCGCGA5500.022.50152627
CGCGTAT3400.022.50021431
TTCGCGC2300.022.493492
TTAACGC6950.022.0080227
GCACTAC107800.021.7696425
ACGATCG3550.021.56781443
ACGCGAA955.549482E-721.31256520
CGACTAT33600.021.0876484
GTCCGTT33700.021.0297011
CCTAACG3000.021.00464238
ACGCGTG3650.020.9658241
Produced by FastQC (version 0.11.5)