FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46360266
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA963440.20781589130657707No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC559760.12074132620377975No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT524980.11323921221677201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA2050.020.868830
TAGGCGA18350.020.38792638
CTAGGCG18850.020.05434437
CGTTGCG10300.019.9306841
AATCGCG6700.019.8023336
TCGCGTA2400.019.6722668
CGATAGG5750.019.54904425
ACGCGAT5500.018.42245731
TAGTCGG13100.018.02424436
TATCGCG6250.017.98622512
CGGTAAT14800.017.36633343
CGGTCGA11450.017.33101539
CGGTATA20800.017.18637511
GTTCGCG4600.017.1063188
GTATGCG24600.017.04908845
ATACGCG3900.016.76795839
TTCGCGA6100.016.21591815
ACGGTAT22500.015.987617510
TAGGCCC31250.015.89848617
GTATACG17100.015.7755546
Produced by FastQC (version 0.11.5)