FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47854381
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA787230.16450531457088538No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAA739560.15454384416758No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTTT588100.12289365941229079No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAG585370.12232317872840105No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC536540.1121193062762634No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACACT511780.10694527633739531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA71900.021.2476523
ACGCTAC72200.021.0035524
TCGACGA199400.020.60187145
CGCTACC77750.019.5325925
CTCGACG212750.019.34026744
ATCGAGC147150.019.11030442
TAAACCG220950.018.72437911
CCCAAAT262700.018.5941751
AACCGAT226400.018.23392713
AAACCGA233700.017.70286812
TCTCGAC231100.017.6488443
ACCGATG235450.017.56173314
ATCTCGA235900.017.51858742
CGCGCAT10150.017.0923731
GAAAACG92350.016.90776820
ATGCGCG13400.016.78905345
TTAAACC248350.016.73990210
AAAACGC93750.016.58352521
TACGTAT38550.016.45815725
CGAGCTT178700.016.39097444
Produced by FastQC (version 0.11.5)