FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50216357
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG698870.13917178420569218No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT573120.1141301428934799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG125150.028.0480986
CGACGAG125900.026.6986948
GTTCGAC135400.025.3099295
CGGTATA185850.024.69654520
ACGAGGA171900.022.63023210
CGAGGAT164900.022.52663611
GTATAGA217350.022.03845822
ATGACGG199300.021.99135616
GACGAGG177850.021.7465639
CAGTTCG162150.021.7034853
TATAGAG224650.021.45255723
AGTTCGA162300.021.4477374
ATAGAGT227250.021.1279124
GGTATAG235450.019.70403321
AACGACG74300.019.68316316
ACGCGTC16250.019.52235440
AGACCGC106600.019.2085194
GATGACG222150.019.1218315
TCGACGA186000.019.1121397
GACCGCG106400.019.096595
Produced by FastQC (version 0.11.5)