FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46839198
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT583480.12457087757992782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT156150.018.7900145
CGTTACG21050.016.99503521
AGACCGC172350.016.8800624
ACCGCGT174250.016.6954156
CAGACCG181000.016.2601743
GACCGCG182150.015.9470255
CCGCGTT185600.015.7109597
CGCGTTC189600.015.3439048
AGAACGA194350.015.17774644
CCCCCCT39000.014.946461
CCCGTAT29350.014.7996821
GTCCGTT33100.014.414881
CTCCGGT54850.013.7458421
CTCCGAT51200.013.6268331
GCGTTCT219300.013.3889819
TCAGACC225250.013.3064132
CCCCCTT109900.013.2498141
CTCCGTT64650.013.2287321
TCGACGA43650.013.09305245
CGCCTGT51450.012.8607151
Produced by FastQC (version 0.11.5)