FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48669314
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT760030.15616205315735496No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA543830.1117398120713187No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG495260.1017602179475963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27250.023.3668546
CGACGAG32250.019.7440858
GTTCGAC33200.019.5857645
GACCGCG276000.018.5379685
ACCGCGT277650.018.443996
AGACCGC285350.018.1434734
CAGACCG287650.018.1003843
CGGTATA49450.017.65383520
CCGCGTT299300.017.1474237
CGCGTTC301500.017.0297648
CGTTACG43200.016.71840721
TTACGCA17200.016.0900348
TCAGACC346300.015.8410622
GCGTTCT328050.015.7611889
ATTACGC16600.015.587277
AGTGCTA88500.014.94884612
CCCGTAT26650.014.8620971
ACGCGTC17300.014.6968540
CGTTCTC371750.014.56195310
CTCAGAC416950.014.5512581
Produced by FastQC (version 0.11.5)