FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48669314
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24900.011.74625226
TAGCACG29150.010.80583229
CGCGTTA6950.010.68223611
ATAGGGG110500.010.34438138
GGCATAG111850.010.13812135
CGGCTAA77550.09.45831831
CGTAACG19450.09.25506142
CTAGAAT112100.08.994711
GGAGGTC169150.08.9016264
CGTGGCG82650.08.7413191
CGACGTA6259.094947E-128.63946824
CGGCTTA47850.08.5107930
GTCACTC169300.08.464558
GCATAGG134700.08.43539536
GGGAGGT187500.08.4145713
CATAGGG135700.08.39009137
CGCTGAT105650.08.3721661
CTATAGT380200.08.3113244
TTCTATA401150.08.2924512
ACGGCTA87800.08.27715730
Produced by FastQC (version 0.11.5)