FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49904600
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG2769710.5550009417969486No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2216900.44422758623453545No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC1413570.2832544494896262No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCT1356640.2718466834720647No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1264810.2534455741554887No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC1185290.23751117131486876No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGT1011340.20265466510101274No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT950400.19044336594221775No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAG871420.17461716955951959No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC845000.16932306841453493No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT830680.16645359345631464No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC770450.15438456575145376No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA762010.15269333889060327No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTC740980.14847929850154096No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG659110.1320739971866321No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT628380.1259162482015686No Hit
TTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC608470.12192663602152909No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG564530.11312183646397327No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA509150.10202466305711297No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGA502400.10067208233309154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT407300.027.5527731
CCGGCTT480300.025.5322512
TCACGCT485700.025.4869888
TTCACGC503850.024.6805157
CACGCTC508250.024.4359159
CGGCTTC518950.024.207453
ACGCTCA536000.023.46461910
AACGCTA524700.023.30953223
ACGCTAC528050.023.14887224
TCGGCGC202300.023.02193545
CGCTACC534650.022.79998825
AAAACGC537950.022.64832521
GAAAACG541600.022.42522420
TTCGGCG213100.021.89740644
AAACGCT560450.021.69496222
AGACCGC94150.020.9597554
CGCTCAG606350.020.67541311
ACCGCGT96000.020.2978746
GACCGCG98150.019.8990945
GTTAGTT314550.019.82763344
Produced by FastQC (version 0.11.5)