FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49904600
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG2390880.47909010391827606No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC1519370.30445489994910285No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA1245380.24955214549360177No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT1153920.23122517763893508No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGA1127890.22600922560244946No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA879180.1761721364363205No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCG839750.16827106118474047No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTT822820.16487858834656524No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT797130.15973076630210442No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCAC751150.1505171867923999No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA701270.14052211619770522No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC689210.13810550530412027No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG624470.12513275329328358No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG582340.116690645752095No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG547120.10963318010764539No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTG540160.10823851909443218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT669800.016.99210544
GCCCGTG671500.016.94523845
TCAGACG172650.014.5553592
CGGCTTA107250.013.971230530
CTGCCCG884100.012.92139343
GGCATAG134150.012.53917135
CGGTTTA59450.011.93169138
ATAGGGG144950.011.83804638
CAGGGGT137300.011.34955540
AGGATTC494650.011.33582845
AACTAAC504000.011.12977737
AGGGATC170900.010.9789382
GGAGGTC197100.010.9006674
AGACGTG233650.010.8227314
CTAACCA514100.010.81475739
TGCGTCG26500.010.77815710
ACTAACC515800.010.73545638
TGGCATA158250.010.72918134
AGCTTAG158600.010.70712245
GTTTCGT72700.010.6976131
Produced by FastQC (version 0.11.5)