FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51064612
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1020470.19983898046655088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT253350.022.3216781
TCACGCT270700.021.9010898
AACGCTA274600.021.31969323
ACGCTAC275550.021.16453724
ACGCTCA291150.020.80326810
CCGGCTT303700.020.6558652
CGCTACC282350.020.61497525
TTCACGC290000.020.5676697
AAAACGC292150.020.09290721
CACGCTC299400.020.0722229
GAAAACG302350.019.35556420
CGGCTTC337250.019.2547783
CGAACGA40950.019.06558832
AAACGCT311050.018.78520422
ACCGCGT64050.017.9512846
TAAACCG130450.017.90317311
ACGAACG45300.017.5824631
GCACTAC129250.017.58186725
CCCAGAT212950.017.4012451
CTCGACG132700.017.29428344
Produced by FastQC (version 0.11.5)