FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51064612
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG528880.10357074680211024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28400.013.866425526
GGGAGGT281950.013.0971873
GGCATAG210300.013.08263435
AGCTTAG213950.012.82556645
GGAGGTC290100.012.4036444
ATAGGGG232200.011.94583438
AGGGATC141100.011.8737052
CGGCTTA103550.011.8459130
TGGCATA235200.011.76444534
TCAGACG109050.011.0121612
ATATAGC43650.010.6653286
ACTCTCC337800.010.46786611
GAGGTCA347200.010.4548315
GAGCTTA266550.010.40644844
CAGGGAT178850.010.3694471
CATAGGG268550.010.36242237
CGGTTTA65250.010.31712338
GTCACTC353950.010.1987278
GCATAGG274100.010.119637536
TCACTCT359100.010.1158499
Produced by FastQC (version 0.11.5)