FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50345495
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG531650.10560031240133799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT202400.018.3412278
AACGCTA193950.018.2937223
ACGCTAC195350.018.27793324
GCCGGCT184850.017.801931
CGCTACC200300.017.7026925
CCGGCTT218850.017.1790122
TTCACGC217400.017.0969247
AAAACGC212400.016.78918821
CACGCTC234300.015.7672449
GAAAACG226350.015.75446620
AAACGCT228350.015.61666822
ACGCTCA239150.015.56037510
GAACGAT146400.015.35290645
CCCGTAT39350.014.9291451
ACCGCGT145750.014.8348486
GACCGCG146800.014.7134145
CTACACG69550.014.65437245
CTCCGAT57150.014.4934161
CGGCTTC282350.014.407183
AGACCGC151500.014.271814
Produced by FastQC (version 0.11.5)