FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50665243
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT120850.021.3112836
GACCGCG121450.021.1875215
AGACCGC127150.020.520754
CAGACCG131950.019.8936043
CCGCGTT131750.019.4793387
CGCGTTC132250.019.1845748
CTCCGGT76550.017.955741
TAAACCG57200.016.59412211
ACGCGTC11400.015.81144540
CTCGACG61300.015.64238744
GTCCGTT36250.015.3904381
CTCGTAT56300.015.3437021
GCGTTCT169950.014.9950359
TTCGACG20500.014.8125776
CTCGCGT10950.014.7919591
CGCAATC43650.014.7384942
CCCGTAT38650.014.5511661
TCGGCGC57150.014.48843345
TCAGACC193150.014.2896362
CTACACG71950.013.89135245
Produced by FastQC (version 0.11.5)