FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47672437
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG3082690.6466399022143551No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG694190.14561663797468544No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG690970.1449411952655158No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT679930.14262539169121982No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA497450.10434750797405218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT520400.032.3141371
CCGGCTT636400.029.1855092
TTCACGC693750.026.9417087
CGGCTTC710750.026.8029843
TCACGCT704350.026.5299788
CACGCTC731600.025.526529
ACGCTCA747400.025.26977210
AACGCTA799250.023.31554823
ACGCTAC802850.023.1830824
CGCTCAG814100.023.15213811
CGCTACC817800.022.74014525
AAAACGC822250.022.72347321
GGCTTCA854050.022.7063224
GAAAACG823000.022.46718820
AAACGCT839600.022.23517222
CTTCACG906750.020.9478156
GCTCAGG891300.020.76300212
GCTTCAC958750.020.3512345
GCTACCT922050.020.31787126
CTACCTC933700.020.22812827
Produced by FastQC (version 0.11.5)