FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46069036
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTTA153000.012.86895330
GTTGTCG31950.012.74632926
TCAGACG93250.012.1350022
GTTTCGT114100.011.701041
TTCGTCG98300.011.6724723
CAGGGGT189000.011.06501740
TAATCGG48550.011.0289684
GGGGTGT193550.010.98759342
TTTCGTC108200.010.9573122
AGGGGTG202700.010.93629141
TCGGCTT182300.010.91169429
AGCTCGG183150.010.822968526
CGTCGAG114150.010.7618525
TGCCCGT121450.010.72555344
GCTCGGC182550.010.674870527
GTAACGA48700.010.57902418
ATAATCG54350.010.4729053
TCGTCGA112800.010.4712224
CGTGGCG155550.010.3633071
GGTGTGT215550.010.11380144
Produced by FastQC (version 0.11.5)