FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52590487
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA111300.018.47637623
ACGCTAC119050.018.23742924
GTCCGTT42000.017.9558091
CGCTACC122350.017.67197825
TTCACGC132850.017.3930877
TAAACCG149650.017.10940711
TCGACGA147400.017.00412445
GACCGCG51550.016.8048485
TCACGCT137550.016.716998
CTCGACG153400.016.42703644
AGACCGC53800.016.3531074
CCGGCTT153900.016.1118982
ACCGCGT53600.016.0781656
AACCGAT163700.015.68217913
CCCAAAT256250.015.38266851
AAAACGC135350.015.21054221
AAACCGA172500.014.93433312
CCCGTAT32350.014.8918591
CTCCGGT63100.014.8056731
TCTCGAC171100.014.78028843
Produced by FastQC (version 0.11.5)