FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52590487
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG34650.014.34821926
CGGCTTA124400.012.80460430
ATATAGC45350.012.4892076
TTCGTCG87000.011.5329483
GTTTCGT96300.011.3773111
TGTCGTT45300.011.32375128
CGGTTTA88000.011.18243938
ATAGGGG135550.010.3828938
GCTCGGC154250.010.31211927
AGCTCGG157400.010.30479826
TAGCACG22750.010.28502129
GGCATAG134600.010.18838735
CGTCGAG99850.010.1837765
TTTCGTC107150.010.1409742
TCGGCTT158750.010.07648729
TATAGCT55700.010.05137357
CAGGGGT167050.09.96175240
GTCGTTC55100.09.84053529
AGGGGTG172050.09.82421941
TTGTCGT56800.09.82329227
Produced by FastQC (version 0.11.5)