FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49422334
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG906530.18342516968138334No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG675790.1367377752738266No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG574570.11625715612702549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT268900.026.6702821
TTCACGC489700.024.9714187
TCACGCT503550.024.2220788
CCGGCTT338700.024.098972
CACGCTC538450.022.673139
ACGCTCA540650.022.6432710
CGGCTTC392100.021.4305463
GACCGCG69250.020.8511335
AACGCTA603850.020.51230623
ACGCTAC606250.020.42745424
CGCTCAG587750.020.35039111
ACCGCGT72050.020.1344496
AGACCGC74550.020.0628384
CGCTACC614700.020.05885125
AAAACGC621000.019.95642721
GAAAACG618350.019.52528820
AAACGCT640200.019.41430922
CTTCACG648500.019.356026
GCTTCAC694350.018.7743245
CAGACCG85200.018.0039583
Produced by FastQC (version 0.11.5)