FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41709546
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGC61900.021.37364245
AGACCGC87400.020.363884
TTCGGCG64950.020.30059444
ACCGCGT88700.020.1405836
GAACGAT92350.019.5158545
CTCCGGT73400.019.4691091
CAGACCG94150.019.0712933
GACCGCG93750.019.0077925
CGCGTTC94100.018.98488
GTCCGTT38950.018.5466691
CCGCGTT96050.018.5290997
CGTACGC77300.017.202275
CGCAATC35100.017.1170652
CCGTACG78500.016.796684
TCCGGTT59700.016.509162
TACGCCA83700.015.9943547
AGAACGA114000.015.84896844
GGGATAA99750.015.6765215
AGACGCT123600.015.60100741
TAATTAT102150.015.550389
Produced by FastQC (version 0.11.5)