FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51304580
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT75300.019.7839836
AACGCTA101850.018.6430723
ACGCTAC102650.018.49777624
GACCGCG81200.018.4296195
AGACCGC83100.018.1716634
CTCGACG99400.017.90301144
CGCTACC108000.017.51895725
TAAACCG103950.016.92489211
TCGACGA106000.016.59727945
CAGACCG92250.016.5400373
GAACGAT92300.016.50148445
CCGCGTT90800.016.4275747
CGCGTTC91600.016.259548
CTCGTAT77250.016.2058661
TCACGCT128050.015.8303668
AAAACGC122200.015.64894321
TCGGCGC55600.015.49744145
TTCACGC133400.015.4821987
GTCCGTT38800.015.4363941
TTCGACG14300.015.4220026
Produced by FastQC (version 0.11.5)