FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43470412
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTG477930.10994374748507099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT29600.016.8424131
GTCGAAA46200.014.8434671
ACGTTCG11200.014.4601766
ACCGCGT61450.013.7999836
AGACCGC61250.013.7948094
CTCCGGT54300.013.2939451
CGAAATA54250.013.2074493
GCGGACT56300.013.1267432
TCGAAAT54650.012.9870932
CGCGCTA2309.6881195E-812.7352094
GTCCGAT23100.012.695071
CCCGTAT27800.012.6585381
CTCGGTT73600.012.4744191
CGCGTTC68950.012.2988988
CCGCGTT69650.012.2721777
CGTTACG12700.012.22080921
TCGACGA26050.012.20166745
TCACGCT92750.012.1016838
CAGACCG73700.011.984233
CCCCCCT34450.011.8520391
Produced by FastQC (version 0.11.5)