FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43470412
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG80550.010.2580735
ATAGGGG80050.010.25439238
AGCTTAG84350.010.12013745
TCGCGCT39000.09.00932745
CATAGGG91750.08.89813437
TCAGACG76100.08.7819952
ATAGCTC56050.08.66790239
GAGCTTA99400.08.46446644
TGGCATA100400.08.41891234
TCACCCG71900.08.4186953
GCATAGG95700.08.37024136
ACCCGGC65650.08.1568255
TAGGCTA60000.08.13136310
GCATTCG49350.08.1162283
CATTCGG52900.08.0820094
GGGAGGT173450.07.9785183
TAGCGTA3951.1329976E-57.971302537
CCGGACA70300.07.867308612
TTAACGG44350.07.86428933
CAGACGT85500.07.79373551
Produced by FastQC (version 0.11.5)