FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48968649
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2615100.5340355622226785No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA571300.1166664818545433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT375900.032.97293545
AGACCGC449600.028.2468094
CAGACCG454300.028.0338133
GACCGCG457700.027.7012985
ACCGCGT459800.027.643396
AGAACGA449350.027.62587744
CCGCGTT472300.026.9164527
TCAGACC484000.026.537132
CGCGTTC479150.026.522268
ATACGGA486750.025.64960533
AATACGG490900.025.4676732
GCGTTCT500050.025.413749
TACGGAG491950.025.38396534
AAGAACG491100.025.2549943
CAATACG501500.024.96345731
ACGGAGA514800.024.35972835
CGTTCTC524400.024.31089810
CCAATAC544050.023.18280630
CCCCAAT555050.022.76243828
ACTCCCC566650.022.31950825
Produced by FastQC (version 0.11.5)