FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48968649
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA1949630.398138408923636No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA619740.12655852523111266No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC609490.12446534924825065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT423950.023.3822332
CACAGAC470400.021.7381531
CAGACTC491550.020.3680023
AGACTCA545800.019.0854824
ACTCAGA542300.018.3291286
GACTCAG588850.017.006255
AACCCAC592300.016.38883816
CCGACAA607600.015.77790740
GCCGACA608850.015.72703939
TGCCGAC622600.015.43031638
AGAACCC629750.015.31060714
CGACAAG625700.015.29632241
TCAGAGA644100.015.2333238
GAACCCA651050.014.89610415
ACCCACC672800.014.65200317
AGAGAGA670100.014.56554910
CTGCCGA667550.014.42500837
AGAGAAC681300.014.18825512
ATAGGGG98250.014.15181138
GAGAGAA705950.013.72746511
Produced by FastQC (version 0.11.5)