FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43484334
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGC61900.017.83249345
ATGCGCG32550.016.8175245
GAACGAT72150.016.76658245
TTCGGCG67400.016.62143344
ACGTTCG22550.016.3604896
TAAACCG45350.016.22119911
AGACCGC77900.015.2314364
TCGACGA50200.015.12282245
CCGCTAT29550.015.0177161
ACCGCGT79400.015.0160056
TGCGCGT27600.014.93649445
GTCCGTT27450.014.7715241
CGTATCG42850.014.4509043
TACGGTA94000.014.21535144
CGTACGC79150.014.08062655
ATCGAGC42700.014.01369842
AGACGCT133750.013.9935741
ACCCGTC27250.013.95149332
CCGTATC52550.013.9259182
CCGTACG79650.013.9074334
Produced by FastQC (version 0.11.5)