FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43484334
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC88500.019.1921395
TCACCCG108100.015.8812453
CGGACAC106250.015.67969413
CCGGACA108300.015.33987512
CGGACAG111600.015.05033719
ACGGACA112800.014.89031218
CACCCGG125750.014.1710784
GCCCGGA123500.013.98086610
GCGTCGT26750.013.782227511
CACGGAC124200.013.50546417
ATAGCTC122750.013.3208439
GACACGG130700.012.90183515
CCCGGAC146300.011.9853211
CGGCCCG143600.011.804838
ACACGGA145300.011.77636716
CGGTTTA40150.011.36991638
TGATAGC144700.011.36089637
TAGCTCT145400.011.33871440
GGATTGA154300.010.93491725
TAGCGTA7000.010.92306737
Produced by FastQC (version 0.11.5)