FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45220509
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC499920.1105516083421352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC122800.020.5741724
GTCCGTT31050.019.656451
AACGCTA130400.019.32281323
CGCGTGT11800.018.895161
AAAACGC136550.018.60076121
TCACGCT146250.018.2734368
GAAAACG137600.018.24627520
TCGGCGC105050.017.73104945
TTCACGC153850.017.4877247
AAACGCT147700.017.1661122
CGCTACC147300.016.99969125
ACGTTCG11450.016.896346
ACGCTCA162800.016.74743710
TTCGGCG115000.016.19735744
ATGTCGC151700.016.1826935
GCCGGCT159550.016.1624091
CTACACG40200.015.83654645
CCGGCTT181250.015.7770222
CACGCTC168950.015.6584669
GTTAGTT98050.015.55584944
Produced by FastQC (version 0.11.5)