FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45894296
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG19250.017.9966896
ACCGCGT69500.017.1551176
AGACCGC70900.016.9252414
CGTTACG18700.016.60513321
ATGCGCG33250.016.5978745
GAACGAT73650.016.39343545
GTCCGTT31950.016.3563541
TCGGCGC90900.016.23137545
CAGACCG76500.015.8938723
CCCGTAT28750.015.8264281
TCGACGA62100.015.41612445
TTCGGCG97450.015.28193444
GACCGCG78150.015.2686545
CTCGACG63500.015.12712444
CGATTAG40450.014.860954
TAAACCG64550.014.81185811
TGCGCGT28000.014.7221445
TACGGTA106600.014.66661144
CCGCGTT81650.014.6298887
CCGTACG101300.014.5352384
Produced by FastQC (version 0.11.5)