FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50121620
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT875880.1747509358237024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC142400.029.2004854
ACCGCGT143900.028.9107556
GACCGCG145300.028.632715
CAGACCG150500.027.733613
CCGCGTT152850.027.1442037
CGCGTTC157150.026.4012878
GAACGAT167650.024.55987245
AATACGG176400.023.35438232
ATACGGA179600.022.93826733
TACGGAG180050.022.84344734
TCAGACC187300.022.7771592
AGAACGA185600.022.25734144
ACGGAGA196150.021.03728135
GCGTTCT199750.020.9519
GACGAAA66550.019.44009445
CTCAGAC261300.019.4364951
CAATACG224250.018.74230631
ATGCGCG47050.018.60238344
TGCGCGT48200.018.2052345
AAGAACG232000.017.83496943
Produced by FastQC (version 0.11.5)