FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46171661
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC598900.12971159950256067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34200.022.6312456
CGGTATA55750.018.52432820
TCGACGA42500.018.2114567
CGACGAG42500.018.1584958
GTTCGAC42550.018.1372745
CGTTACG30600.017.72026621
GACCGCG111250.017.0694645
ACCGCGT113500.016.8896186
AGACCGC115150.016.5501384
GTCCGTT33000.016.5037941
CAGACCG123250.015.7919353
CCGCGTT126150.015.2137737
CTCGGTT90000.015.1284781
AGACGCT293600.014.97496441
GCTCGGC258100.014.75929845
AAGCGGC277150.014.62087928
GTATAGA79000.014.52505522
ACGCGTC14500.014.4313240
GAACGAT139200.014.40241345
CCCCCCT48250.014.3193541
Produced by FastQC (version 0.11.5)