FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46171661
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG160000.012.93778438
GGCATAG163600.012.47287735
AGCTTAG169650.012.33339445
GTCACTC231150.012.0796528
GGGAGGT257500.011.6943593
GGTCACT248950.011.1355427
GAGCTTA187850.011.07880644
GGAGGTC262250.011.0103744
TGGCATA191500.010.71464234
AGGTCAC277500.010.5575496
ACTCTCC275950.010.43544411
CATAGGG203550.010.19180137
TCACTCT286850.010.0939949
GCATAGG204950.010.06683136
CACTCTC289100.09.73487110
GAGGTCA312750.09.5774425
TAGCACG17400.09.309529
CGTAACG18900.09.28602942
GTTGTCG23650.08.84749526
TAGGGGA323000.08.763350539
Produced by FastQC (version 0.11.5)